TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or description | Reference |
|---|---|---|
| E. coli S17-1λpir | recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 | 57 |
| V. cholerae strains | ||
| SIO | Wild type | 53 |
| TP | Wild type | 53 |
| N16961 | Wild-type O1 El Tor | 22 |
| S2151 | SIO ΔmshA | This study |
| S2153 | SIO ΔvpsL | This study |
| S2163 | SIO ΔpepN | This study |
| S2164 | SIO ΔVC2215 | This study |
| S2101 | SIO ΔtnaA | This study |
| S2123 | SIO ΔpomA | This study |
| S2104 | SIO ΔflaA | This study |
| T2114 | TP Δzur | This study |
| T2151 | TP ΔmshA | This study |
| T2153 | TP ΔvpsL | This study |
| T2127 | TP ΔVC1437 | This study |
| T2112 | TP ΔlacI | This study |
| T2101 | TP ΔtnaA | This study |
| T2130 | TP ΔVCA0859 | This study |
| T2123 | TP ΔpomA | This study |
| T2104 | TP ΔflaA | This study |
| Plasmids | ||
| pRL27 | Tn5-Rl27 oriR6K Kmr | 32 |
| pFL122 | RSF1010 derivative, lacZ MCS mob Smr | 33 |
| pFL122L | Linker containing SacI site ligated into BglII site of pFL122 | This study |
| pBSL181 | oriR6K Apr Cmr | 1 |
| pFLcm | pFL122L containing cat gene from pBSL181 | This study |
| p519ngfp | RSF1010 derivative containing nptII promoter before gfp gene, mob Kmr | 39 |
| pFLgfp | pFLcm122L containing PCR amplicon of nptIIp-gfpmut2 of p519ngfp | This study |
| pGP704sac28 | pGP704 derivative containing sacB gene of B. subtilis oriR6K, mob Apr | 6 |
| pMB2190 | pBR327 derivative, Kmr Apr | 3 |
| pGPKm | pGP704sac28 containing Kmr gene of pMB2190 | This study |
| pGPVX0408 | ΔmshA in pGPKm | This study |
| pGPVX0892 | ΔpomA in pGPKm | This study |
| pGPVX0934 | ΔvpsL in pGPKm | This study |
| pGPVX1494 | ΔpepN in pGPKm | This study |
| pGPVX2188 | ΔflaA in pGPKm | This study |
| pGPVX2215 | ΔVC2215 in pGPKm | This study |
| pGPVCX0161 | ΔtnaA in pGPKm | This study |
| pGPVX0378 | Δzur in pGPKm | This study |
| pGPVX1437 | ΔVC1437 in pGPKm | This study |
| pGPVX2337 | ΔlacI in pGPKm | This study |
| pGPVCX0859 | ΔVCA0859 in pGPKm | This study |