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Aerobic growth (OD600) and acidification of L. lactis with and without hemea
| Membrane preparation | OD600 | pH |
|---|---|---|
| Wild type + heme | 5.25 | 5.04 |
| Wild type | 2.60 | 4.34 |
| CydAΔ + heme | 2.57 | 4.33 |
| CydAΔ | 2.66 | 4.27 |
| CydAΔ + pIL253CydABCD + heme | 5.07 | 5.06 |
| CydAΔ + pIL253CydABCD | 2.62 | 4.30 |
L. lactis wild-type, CydAΔ, and CydAΔ cells, complemented with plasmid pIL253CydABCD (carrying the cydABCD operon), were grown aerobically overnight in M17G medium at 30°C in the presence or absence of heme.
