TABLE 4.
Relative drop in fluorescence of early-exponential-phase, mid-exponential-phase, and overnight (or late-stationary-phase) cultures of DCCD-treated and untreated wild-type and CydAΔ cells after glucose additiona
| Strain | OD600 (phase) | % Fluorescence drop
|
|
|---|---|---|---|
| −DCCD | +DCCD | ||
| Wild type | 0.48 (early) | 60.7 | 26.7 |
| CydAΔ | 0.4 (early) | 40.0 | 5.1 |
| Wild type | 1.08 (early/mid)b | 71.8 | 37.2 |
| CydAΔ | 1.04 (early/mid)b | 58.6 | 4.9 |
| Wild type | 1.53 (mid) | 38.0 | 22.1 |
| CydAΔ | 1.49 (mid) | 46.9 | 7.0 |
| Wild type | 4.49 (O/N)c | 46.9 | 63.1 |
| CydAΔ | 2.6 (O/N)c | 45.7 | 5.7 |
The percent fluorescence drop compares two fluorescence values in one trace at one time point with a maximal difference in value: the low trace value is the measured level of fluorescence after the addition of glucose; the high trace value was extrapolated from the fluorescence recording before the addition of glucose. Experimental conditions are described in Materials and Methods.
These fluorescence traces are described in detail in the legend of Fig. 2.
Cultures grown overnight (O/N) were grown for more than 20 h and considered (late) stationary-phase cells.