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Oxygen consumption by membrane vesicles at 25°C after the addition of NADH or NADa
| Membrane preparation | Addition | mean μmol O2 consumption/min/mg protein ± SD |
|---|---|---|
| Wild type + heme | NADH | 42.18 ± 0.12 |
| Wild type | NADH | 6.62 ± 0.35 |
| CydAΔ + heme | NADH | 8.41 ± 1.32 |
| Wild type + heme | NAD | ND |
Membrane vesicles were prepared from the different cells as described in Materials and Methods. ND, no consumption of oxygen detected after the addition of NAD.
