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Experimental analysis of the putative PhoR consensus binding motif. Based on sequences 1 and 2 in Fig. 7, an artificial 19-bp consensus sequence consisting of two direct 8-bp repeats of the sequence ACTGTGAA and the spacer AAT was inserted between two fragments that were not bound by PhoR∼P. The resulting fragment and mutated derivatives were tested in gel mobility shift assays for the ability to bind PhoR∼P (for details, see the legends to Fig. 3 and 4).
