TABLE 1.
Bacterial strains and plasmids used in this study
| Strains | Relevant characteristics | Source/reference |
|---|---|---|
| E. coli DH5α | F−thi-1 endA1 hsdR17(r− m+) supE44 ΔlacU169 (φ80lacZΔM15) recA1 gyrA96 relA1; host for cloning procedures | Invitrogen |
| E. coli BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (λcIts857 ind1 Sam7 nin5 lacUV5-T7 gene 1); host for overproduction of plasmid-encoded recombinant proteins | Novagen |
| C. glutamicum ATCC 13032 | Biotin-auxotrophic wild-type strain | 1 |
| Plasmids | ||
| pET16b | Ampr; PT7lacI oriV from pBR322; E. coli expression vector for overproduction of proteins with an N-terminal decahistidine tag that can be cleaved off by factor Xa | Novagen |
| pET16b-PhoR | Ampr; pET16b derivative for overproduction of PhoR with an N-terminal decahistidine tag (PhoRN-His10) | This work |
| pMal-c | Ampr; PtaclacIq ColE1 oriV; E. coli expression vector for construction and overproduction of fusion proteins containing the MBP of E. coli (MalE) without its signal peptide | New England Biolabs |
| pMBP-PhoSΔ1-246 | Ampr; pMal-c derivative for overproduction of the kinase domain of C. glutamicum PhoS (amino acid residues 247 to 485) fused to the C-terminus of the E. coli MBP without signal peptide (MBP-PhoSΔ1-246) | This work |
| pET2 | Kanr; promoter-probe vector | 27 |
| pET2-phoR1 | Kanr; pET2 with a 233-bp fragment of the phoR promoter | This work |
| pET2-phoR2 | Kanr; pET2 with a 200-bp fragment of the phoR promoter | This work |
| pET2-phoR3 | Kanr; pET2 with a 180-bp fragment of the phoR promoter | This work |