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. 2007 May 4;189(14):5257–5264. doi: 10.1128/JB.00254-07

FIG. 2.

FIG. 2.

Panel A shows the scheme to fuse in the chromosome of the wild-type of C. glutamicum (Cg) six His codons at the N-terminal end with accD1 to result in chromosomally encoded H6-AccD1 in the H6D1 strain. Panel B shows growth of the resulting strain on LB broth (⋄) compared to the wild type (▴). Also shown is growth of a similarly constructed strain (H6D2 strain) with six His codons fused in the chromosome at the 5′ end of accD2 (▴). Panel C shows the isolation of AccBC (upper arrow) together with H6-AccD1 via affinity chromatography (lower arrow).