TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristics or use | Reference or source |
|---|---|---|
| C. glutamicum strains | ||
| ATCC 13032 | Wild type | Culture collection |
| H6D1 strain | Chromosomal fusion of H6 codons at the N terminus of accD1 | This work |
| H6D2 strain | Chromosomal fusion of H6 codons at the N terminus of accD2 | This work |
| Δpyc strain | C. glutamicum deleted of biotinylated pyruvate carboxylase | 26 |
| paccBC p-H6D2-E strain | C. glutamicum with pJC1accBC and pEKEx3-H6D2-accE | This work |
| paccBC p-H6D3 strain | C. glutamicum with pJC1accBC and pEKEx3-H6D3 | This work |
| Δpyc paccBC p-H6D1 strain | C. glutamicum Δpyc with pJC1accBC and pEKEx3-H6D1 | This work |
| Δpyc paccBC p-H6D1-E strain | C. glutamicum Δpyc with pJC1accBC and pEKEx3-H6D1-accE | This work |
| Δpyc paccBC p-H6D2-E-D3 strain | C. glutamicum Δpyc with pJC1accBC and pEKEx3-H6D2-accE-D3 | This work |
| Plasmids | ||
| pJC1accBC | Overexpression of biotinylated α-subunit | This work |
| pEKEx3-H6D1 | Overexpression of H6-accD1 | This work |
| pEKEx3-H6D2-accE | Overexpression of H6-accD2, accE | This work |
| pEKEx3-H6D3 | Overexpression of H6-accD3 | This work |
| pEKEx3-H6D2-accE-D3 | Overexpression of H6-accD2, accE, and accD3 | This work |
| pEKEx3-H6D1-accE | Overexpression of H6-accD1 and accE | This work |
| pJC1 | Shuttle vector | 23 |
| pEKEx3 | Shuttle or expression vector | 13 |
| pK19mobsacB | Vector for integration or deletion | 31 |
| pK19mobsacB-H6accD1 | Allelic exchange of accD1 for H6-accD1 | This work |
| pK19mobsacB-H6accD2 | Allelic exchange of accD2 for H6-accD2 | This work |