TABLE 3.
1,2-cis-DCE epoxide and TCE epoxide formation and DCE epoxide consumption by BMOa
| Strain | 1,2-cis-DCE
|
1,2-cis-DCE epoxide formed (nmol)c | 1,2-cis-DCE epoxide degraded (nmol)d | TCE
|
TCE epoxide formed (nmol)e | ||
|---|---|---|---|---|---|---|---|
| Initial concn (μM)b | Amt degraded (nmol) | Initial concn (μM)b | Amt degraded (nmol) | ||||
| Rev WT | 25 | 57 ± 6 | 57 ± 8 | 48 ± 11 | 80 | 265 ± 14 | 28 ± 6 |
| 75 | 152 ± 8 | 149 ± 13 | 61 ± 13 | ||||
| F321Y | 25 | 60 ± 13 | 60 ± 13 | 55 ± 14 | 80 | 300 ± 15 | 15 ± 5 |
| 75 | 159 ± 10 | 131 ± 9 | 54 ± 9 | ||||
a
The values represent data from at least three separate experiments (means ± standard deviations).
b
Initial liquid phase chlorinated ethene concentration.
c
Reaction vials were quenched with benzene following 10 min of exposure to 1,2-cis-DCE, and 1,2-cis-DCE epoxide was measured colorimetrically as described in Materials and Methods.
d
Reaction vials were quenched with benzene at appropriate time points to follow disappearance of 1,2-cis-DCE epoxide.
e
Reaction vials were quenched with benzene following 6 min of exposure to TCE, and TCE epoxide was measured colorimetrically as described in Materials and Methods.