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NTHi strains R2866 and 86-028NP express vapBC-1 as an operon. Reverse transcriptase PCRs with a forward primer in vapB-1 and a reverse primer in vapC-1 electrophoresed on a 0.8% agarose gel. Lanes 1 and 4, 1-kb DNA ladder; lanes 2 and 5, R2866 and 86-028NP with reverse transcriptase (+RT); lanes 3 and 6, R2866 and 86-028NP without reverse transcriptase (−RT).
