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VapB-1 homodimerizes in vivo. With no protein fused to the LexA DBD, the repressor cannot form a dimer, and transcription of the lacZ reporter gene is constitutive (gray bar). However, when the LexA DBD is fused to VapB-1, competent dimers are formed, and the chimeras can bind to the LexA operator sequence, repressing transcription of the reporter gene (black bar). This level of repression indicates strong VapB-1 homo-interaction.
