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. 2007 May 11;189(14):5041–5048. doi: 10.1128/JB.00290-07

FIG. 7.

FIG. 7.

VapC-1 is an RNase toxin. (A) Total RNA from E. coli K-12 or H. influenzae strain 86-028NP was used as the substrate in RNase activity assays with increasing amounts of the purified VapC-1 toxin. Lanes 1 and 4, MagneHis protein elution buffer control; lanes 2 and 5, 0.35 μg of VapC-1; lanes 3 and 6, 0.7 μg of VapC-1. (B) The Cat protein was cloned into pET24b and purified in the same manner as VapC-1 as a control for any copurifying RNase activity. Lane 1, MagneHis protein elution buffer control; lane 2, 0.35 μg of Cat protein; lane 3, 0.35 μg of VapC-1. Densitometry indicated that the observed RNase activity was specific to VapC-1. Ave, average.