TABLE 1.
Bacteria and plasmids used in this study
| Strain or plasmid | Description | Source |
|---|---|---|
| Strains | ||
| H. influenzae | ||
| 86-028NP | Otitis media isolate from a pediatric patient | R. S. Munson, Jr. |
| R2866 | Blood isolate from an immunocompetent child with meningitis immunized with the Hib vaccinea | A. L. Smith |
| E. coli | ||
| BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (DE3) | EMD Bioscience |
| DH5α | λ− φ80ΔlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | Laboratory collection |
| K-12 | Feces isolate from a diphtheria convalescent | ATCC |
| SU101 | lexA71::Tn5(Def) sulA211 Δ(IacIPOZYA)169/F lacIqlacZΔM15::Tn9 | M. Granger-Schnarr |
| Plasmids | ||
| pACYC184 | Source of cat gene | Laboratory collection |
| pDD676 | pTrcHisA with vapB-1 | This work |
| pDD677 | pTrcHisA with vapC-1 | This work |
| pDD686 | pET24b with vapBC-1 | This work |
| pDD687 | pSR658 with vapB-1 fused to the LexA DBD | This work |
| pDD689 | pET24b with cat | This work |
| pDD690 | pET24b with vapB-1 | This work |
| pDD693 | pMC1403 with R2866 vapBC-1 promoter fusion | This work |
| pDD694 | pMC1403 with 86-028NP vapBC-1 promoter fusion | This work |
| pET24b | C-terminal His tag expression vector | EMD Bioscience |
| pMC1403 | Promoter::lacZ fusion vector | 5 |
| pSR658 | LexA DBD fusion vector | Laboratory collection |
| pTrcHisA | Expression vector with N-terminal polyhistidine tag | Invitrogen |
a
Hib, H. influenzae type b.