TABLE 2.
Transcription activation by His6-RhaS-CTD and His6-RhaR-CTD
| Promoter fusiona | β-Galactosidase sp act (Miller units)b
|
His6-RhaS-CTD or His6-RhaR-CTD activation (fold)c | |
|---|---|---|---|
| Vector only | His6-RhaS-CTD or His6-RhaR-CTD | ||
| Φ(rhaB-lacZ)Δ84 | 0.021 | 21 (His6-RhaS-CTD) | 1,000 (His6-RhaS-CTD) |
| Φ(rhaT-lacZ)Δ84d | 0.29 | 51 (His6-RhaS-CTD) | 180 (His6-RhaS-CTD) |
| Φ(rhaS-lacZ)Δ85 | 0.41 | 0.82 (His6-RhaR-CTD) | 2.0 (His6-RhaR-CTD) |
a
Each strain carried a single-copy lacZ fusion integrated into the chromosome as a λ lysogen and also Δ(rhaSR).
b
β-Galactosidase activity was determined as described in Materials and Methods. The vector-only sample was pSU18. His6-RhaS-CTD was expressed from pSE271, and His6-RhaR-CTD was expressed from pSE272.
c
Activation values (fold) were calculated by dividing the activity in the presence of His6-RhaS-CTD or His6-RhaR-CTD by the activity in the presence of vector only.
d
Φ(rhaT-lacZ)Δ84 is a transcriptional fusion. All other lacZ fusions in this study were translational fusions.