TABLE 3.
Transcription activation by RhaS-CTD compared to full-length RhaS
| Promoter fusiona | β-Galactosidase sp act (Miller units)b
|
Activation (fold) with:c
|
|||
|---|---|---|---|---|---|
| Vector | RhaS-CTD | RhaS | RhaS-CTD | RhaS | |
| Φ(rhaB-lacZ)Δ66 | 0.015 | 34 | 3.5 | 2,300 | 230 |
| Φ(rhaB-lacZ)Δ84 | 0.015 | 36 | 110 | 2,400 | 7,300 |
| Φ(rhaB-lacZ)Δ110 | 0.018 | 91 | 700 | 5,100 | 39,000 |
a
Each strain carried a single-copy lacZ fusion integrated into the chromosome as a λ lysogen and also Δ(rhaSR).
b
β-Galactosidase activity was determined as described in Materials and Methods. All cultures were grown in the presence of l-rhamnose. The vector-only sample was pSE262. RhaS-CTD was expressed from pSE268, and RhaS was expressed from pSE265.
c
Activation (fold) values were calculated by dividing the activity in the presence of RhaS-CTD or RhaS by the activity in the presence of vector only.