FIG. 2.

Effect of overproduction of E. coli (E.c.) or P. aeruginosa (P.a.) PBP 2 on growth and morphology of E. coli BL21. (A) Growth curves of E. coli BL21 incubated in LB at 37°C following IPTG induction of (▴) no plasmid, WT control; (⧫) pET30a(+) with no insert; (•) pACBL18 encoding wild-type E.c. PBP 2; or (▪) pACJW2 encoding wild-type P.a. PBP 2. Inserts: Associated Western immunoblots assaying overproduction of respective PBPs with time. (B) Phase-contrast micrographs of representative cells sampled at the times indicated from the respective cultures overproducing the two wild-type PBPs. Cells were prepared for microscopy as described in Materials and Methods. Bar, 2 μm.