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Effect of PBP 2 overproduction on cellular morphology of E. coli BL21. Cells harboring (A) pET30a(+) with no insert (control), (B) pACBL8 (E. coli PBP 2), or (C and D) pACJW2 (P. aeruginosa PBP 2) were induced with IPTG and cultured in LB. Following 3 h (B and C) or 5 h (A and D) of incubation at 37°C, cells were harvested and prepared for SEM as described in Materials and Methods. Bar, 1 μm.
