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. 2007 May 18;189(14):4975–4983. doi: 10.1128/JB.00207-07

FIG. 5.

FIG. 5.

Effect of overproduction of E. coli (Ec) or P. aeruginosa (Pa) PBP 2 on growth and morphology of E. coli mutant strain MHD79 lacking six lytic transglycosylases. (A) Growth curves of cells grown in LB at 37°C following addition of 1 mM IPTG. +, E. coli MHD79 (control); the DE3 lysogenized MHD79 strain harboring (▴) no plasmid (control), (⧫) pET30a(+) with no insert (control), (•) pACBL29 encoding wild-type E. coli PBP 2, or (▪) pACCA11 encoding WT P. aeruginosa PBP 2 is shown. Inserts, associated Western immunoblots assaying time course of overproduction of respective PBPs from pACBL29 and pACCA11. (B) Phase-contrast micrographs of representative cells from 3-h cultures of E. coli MHD79 harboring (B) no plasmid, (C) pET30a(+) with no insert, (D) pACBL29 (E. coli PBP 2), or (E) pACCA11 (P. aeruginosa PBP 2). Bar, 2 μm.