TABLE 2.
Oligonucleotide primers used in this study
| Primer | 5′-to-3′ sequence (restriction site) | Description | Construct |
|---|---|---|---|
| PBP2-NTerm1 | CTTTAAGAAGGAGATATACATATGCCGCAG (NdeI) | Amplification of n-PB of P. aeruginosa PBP 2 | pACBL17 |
| PBP2-NTerm2 | CCCGAAGCTTGCTCAGGACGATGTC (HindIII) | Amplification of n-PB of P. aeruginosa PBP 2 | pACBL17 |
| EcPBP2-5 | CCCGAAAACGCAGCATATGAAACTACAGAACTC (NdeI) | Amplification of E. coli pbpA | pACBL18 |
| EcPBP2-2 | CCGTCAAAGCTTATGGTCCTCCGCTG (HindIII) | Amplification of E. coli pbpA | pACBL18 |
| EcPBP2-SA-SDM1 | TCCTCCCGCGGCTACAGTTAAAC | SDM of Ser→330Ala on E. coli pbpA | pACBL19 |
| EcPBP2-SA-SDM2 | GTTTAACTGTAGCCGCGGGAGGA | SDM of Ser→330Ala on E. coli pbpA | pACBL19 |
| EcPBP2-1 | GAAAACGCAGCATATGAAACTACAGAACTC (NdeI) | Amplification of n-PB of E. coli PBP 2 | pACBL24 |
| EcPBP2nPB-1 | GAGTTTGAGAAGCTTCGTCAGGTAAATATC (HindIII) | Amplification of n-PB of E. coli PBP 2 | pACBL24 |
| PaPBP3-1 | GGTGGCCATATGAAACTGAATTATTTCCAGG (NdeI) | Amplification of P. aeruginosa ftsI (pbpB) | pACSR1 |
| PaPBP3-2 | GGCCCTCGAGGCCACGCCCTCC (XhoI) | Amplification of P. aeruginosa ftsI (pbpB) | pACSR1 |