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CcpA binding to the pts promoter. Electrophoretic mobility shift assays were used to determine if the point mutation identified in BgaAC resulted in altered binding of CcpA. Purified CcpA (15 ng) incubated with DIG-labeled D39 or BgaAC pts promoter DNAs was electrophoresed on a 5% nondenaturing acrylamide gel. The labeled DNA was detected by chemiluminescence.
