TABLE 2.
ORF and primera | Sequenceb | Positionc |
---|---|---|
spr0562-0565 | ||
F-spr0562 | dTTGATTGAAAGGGTTAGTATTGAC | 11-34 |
F-PTSpro2 | CTAGCTTCCTAGTTTACTCTTTG | 266-288 |
R-PTSpro3 | CAAAGAGTAAACTAGGAAGCTAG | 288-266 |
R-spr0562 | dCTATATGAAACCGTTGTCAATTAC | 429-406 |
R-PTSpro | dCTAATACCATAAGTTTTCCCTTC | 483-461 |
F-PTS3 | dGGAATTTCTAGGAAAGGACTTGC | 647-669 |
R-PTS2 | dCGATAACTGGGATACCTGGTTC | 1201-1180 |
F-UpBgaA | GCTACAGCAGCTATCGTTCTTG | 1818-1839 |
R-UpBgaA | eTATTTTGCTTTTGCTGCGTACTC | 2815-2793 |
F-bgaApro | eTGCGCTCCTATAAAATATAAACTC | 2818-2840 |
BgaA2 | CTACGATACCAAAGTAAGAGCT | 5329-5308 |
F-DownBgaA | eGTGCAGGATTAGTAGTTACTAAAG | 9767-9790 |
DBgaA3 | GTCCTAAATAGAAGATAAAGAG | 9854-9875 |
spr0566-0579 | ||
DBgaA4 | GAACGAACGCTATCAAAACTTGAAAGC | 183-157 |
R-DownBgaA | CAGTTCCTTCTTACCACAAGACC | 762-741 |
spr1806-1817 | ||
F-UpCcpA1 | dACATATGCTGGTCCTCTACCAG | 5336-5357 |
R-UpCcpA2 | fGAAAAAATCAGGGAATCGAGAAG | 6370-6348 |
F-CcpAORF | gTTTACGTTTTCGTGTTGAG | 6374-6392 |
R-CcpAORF | hATGTGTGAGATAGAAAGG | 7411-7394 |
F-DownCcpA3 | fTCTTTTACAAGTAGAGGTACTGATTG | 7412-7437 |
R-DownCcpA4 | dCATCCAACGGAAGTGCAAGTTC | 8451-8433 |
Primers are from the R6 genome. Open reading frame (ORF) designations are given for the regions containing the groups of primers. Forward and reverse primers are represented by F and R, respectively.
Sequences are from the complete R6 genome (15).
Nucleotide positions for primers are listed in the forward or reverse orientation, as necessary.
Primer also contains an EcoRI restriction site and additional nucleotides at the 5′ end (GGAATTCC; restriction site underlined).
Primer also contains a KpnI restriction site (GGTACC) at the 5′ end.
Primer also contains a BglII restriction site and additional nucleotides at the 5′ end (GAAGATCT; restriction site underlined).
Primer also contains an XhoI restriction site and additional nucleotides at the 5′ end (CCGCTCGAG; restriction site underlined).
Primer also contains an NdeI restriction site and additional nucleotides at the 5′ end (GGAATTCCATATG; restriction site underlined).