FIG. 3.

RT-PCR analysis of expression of cinA and cinQ in P. putida KT2440. Electrophoresis was performed on a 2% agarose gel stained with ethidium bromide. Lane bp, 100-bp ladder; lane 1, RT-PCR product from RNAs extracted from CuCl₂-induced cells (after DNase I treatment), using RT 5′ sense and RT 3′ antisense primers; lane 2, negative control for RT, obtained by PCR amplification of the RT product obtained when the reverse transcriptase was omitted, to verify that no genomic contamination was present in the RNA extract; lane 3, PCR negative control obtained by omitting the genomic DNA from the PCR; lane 4, PCR positive control obtained using genomic DNA from P. putida KT2440 as the template.