FIG. 4.

Effect of absence of UmuC-like protein on DNA damage tolerance of S. uberis. (A) Survival of S. uberis ATCC BAA-854 and EH58 after UV exposure. ATCC BAA-854 (1 and 3) and EH58 (2 and 4) cultures were grown exponentially in THY and at an OD₆₀₀ of 0.1, cultures were diluted, and 10 μl of each dilution was spotted on two TSYE agar plates. Plates either were not exposed (−UV) or were exposed to UV light of 50 J/m² (+UV) prior to incubation overnight at 37°C. The results presented are representative of several independent experiments. (B) Sensitivity of S. uberis ATCC BAA-854 and EH58 to mitomycin C (MMC). ATCC BAA-854 (1 and 3) and EH58 (2 and 4) cultures were grown overnight in THY, and 10 μl of overnight cultures and appropriate dilutions were spotted on TSYE agar plates without and with 25 ng/ml of MMC. Plates were grown overnight at 37°C. The results presented are representative of several independent experiments.