TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Relevant phenotype(s) or genotype(s) | Source or reference |
|---|---|---|
| Strains | ||
| S. uberis | ||
| ATCC BAA-854 (0140J) | Genome sequencing strain (sequence available in http://www.sanger.ac.uk/Projects/S_uberis/) | ATCC |
| EH 78 | ATCC BAA-854 derivative containing pEH55, Tetr | This work |
| EH 58 | ATCC BAA-854 derivative ΔumuC (1.4-kb deletion) Tets | This work |
| EH 79 | EH58 containing pEH79, Tetr | This work |
| EH 80 | EH58 derivative, Tets, with umuC complement | This work |
| E. coli | ||
| JM109 | endA1 recA1 gyrA96 thi hsdR17(rK− mK+) relA1 supE44 Δ(lac-proAB) [F′ traD36 proAB laqIqZΔM15] | Promega |
| EH55 | JM109 containing pEH55 | This work |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10(Tetr)]c | Stratagene |
| Plasmids | ||
| pGhost8 | Tetr, a thermosensitive derivative of pGK12 | Maguin et al. (36) |
| pEH55 | Tetr, pGhost with 1.0-kb XbaI-PstI-SalI fragment containing 1.4-kb in-frame deletion of umuC | This work |
| pEH79 | Tetr, pGhost with 3.0-kb XbaI-EcoRI fragment containing hdiR-umuC-ORF3-ORF4 operon without the upstream region | This work |
| pBluescript-II SK+ | Amr, cloning vector | Stratagene |
| pQE-30 | Amr, vector for overexpression of proteins with N-terminal His6 tag | QIAGEN |
| pQE-hdiR | Amr, pQE derivative for overexpression of His-tagged HdiR | This work |
| pBluescript-IR | Amr, E. coli vector carrying 26-bp fragment containing the IR preceding the hdiR | This work |
| pBluescript-ctrl | Amr, E. coli vector carrying 26-bp fragment from the region preceding the hdiR (not containing the IR) | This work |