. 2007 May 18;189(14):5075–5081. doi: 10.1128/JB.00323-07

TABLE 1.

DNA primers

Target gene (primer name)	Sequence (5′-3′)	Purpose^a
pkn30	CTTCTCGAGATGAATACGTATGTAGAT	Mutant construction
pkn30	CTTCTGCAGCTTGTCCATTTTTACCTA	Mutant construction
pkn44	CTTCTGCAGATGGATACTGCAAATTCC	Mutant construction
pkn44	CTTCTCGAGGTGTACAGGTGAAACTTC	Mutant construction
rnpB	ATAGTACCACAGAAAAATACCG	RT-PCR
rnpB	AAGCCGGGTTCTGTTCTCTG	RT-PCR
hglC	TTGTTGCTAACAGTCTGG	RT-PCR
hglC	TTCACTCATTTGCTGACT	RT-PCR
hglD	CACTATCTGTGGACAGGT	RT-PCR
hglD	GATGGGAGTTTGTACCAC	RT-PCR
hglE_A	CCGTAGTCAACCAAGTTG	RT-PCR
hglE_A	TATACTGACTGGCTGCCA	RT-PCR
hglK	TGATGATGCGATCGCTGA	RT-PCR
hglK	CTACGGACAACGTGGATG	RT-PCR
devA	GGTGTTGAGTTGTCAGGT	RT-PCR
devA	GTTTGGGAATCACGAGCT	RT-PCR
devB	TGACTGCGACTACTGCTA	RT-PCR
devB	ATGTCAACCACTACCTTG	RT-PCR
devC	CAGGAGTCCAGGAGAATT	RT-PCR
devC	GGTCAGCAGAACGTAGTT	RT-PCR
devH	TGCAATCTCCATCCTCCT	RT-PCR
devH	CTGTTGATTGACTCTGCT	RT-PCR
asr5349	AACTAAATCTAGTGAGCATGG	RT-PCR
asr5349	AAGCATATAAAAGGTGATGGT	RT-PCR
asr5350	ATGGCATTCATCAAGATACAG	RT-PCR
asr5350	AACGCTTGACTCTTGATATTG	RT-PCR
nifD (A1)	GTATCTCTCTACGCTTGCT	PCR
nifD (A2)	TTACGTGAAATCGCAGCTA	PCR
nifD (A3)	CCGTCTTCAGTCCATCCAA	PCR
rnpB	CCAGTTCCGCTATCAGAGAG	qRT-PCR
rnpB	GAGGAGAGAGTTGGTGGTAAG	qRT-PCR
nifH	CTATTAGACGACGACACCAAG	qRT-PCR
nifH	GCTTCTGCGGGCTTACC	qRT-PCR
asr5349	TAGTGAGCATGGCAGAAATAAC	qRT-PCR
asr5349	AGGCAGGAGGCGGTAAG	qRT-PCR
asr5350	GCTACTCCTCAGTTGCCATC	qRT-PCR
asr5350	AACGCTTGACTCTTGATATTGTG	qRT-PCR

qRT-PCR, real-time quantitative PCR.