TABLE 1.
Growth around wells containing either holo-HasA (10 μM) or heme (10 μM) of strain C600ΔhemA harboring pFR2 derivatives encoding wild-type HasR and its mutantsa
| Protein | Growth
|
|||
|---|---|---|---|---|
| Without dipyridyl
|
With dipyridyl
|
|||
| Holo-HasA | Heme | Holo-HasA | Heme | |
| HasRΔL1 | − | −/+ | + | + |
| HasRΔL2 | − | + | − | + |
| HasRΔL3 | − | + | − | + |
| HasRΔL4 | − | + | + | + |
| HasRΔL5 | − | − | − | −/+ |
| HasRΔL6 | − | + | − | + |
| HasRΔL7 | − | −/+ | − | −/+ |
| HasRΔL8 | − | + | − | + |
| HasRΔL9 | − | + | − | + |
| HasRΔL10 | − | + | − | + |
| HasRΔL11 | − | + | − | + |
| HasR | − | + | + | + |
a
Holo-HasA was used at 10 μM, and heme was used at 10 μM. In all cases, plates contained arabinose (20 μg/ml) without or with 0.2mM dipyridyl. Growth was observed after overnight incubation at 37°C. + indicates growth, − indicates no growth, and −/+ indicates very reduced growth.