FIG. 3.

Effects of single-base-pair substitutions on the ompA promoter −7 region in F. johnsoniae. Promoter activities were determined as described in Materials and Methods. Reactions were performed in triplicate, and standard deviations are marked by error bars. Results for each deletion promoter clone were normalized to a promoter activity of 100% for the wild-type (W.T.) ompA clone (Fj29). The sequences and the numbers indicate the base pairs at the specific positions in the wild-type ompA promoter. N.C., negative control.