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Transcription start site of cexEp. Primer extension was used to map the Rns-independent transcription start site of cexEp with reverse transcriptase and RNA harvested from strain GPM1070/ pGPMRns (Rns+) growing logarithmically in LB medium. A dideoxy chain-terminated sequencing ladder was generated with the same primer used for reverse transcription. The first nucleotide of the cexE mRNA is underlined, and the wavy arrow indicates the direction of transcription.
