FIG. 1.

Analysis of twitching motility. Assays were carried out on 1% TAS agar with stab-inoculated D. nodosus cultures incubated for 5 to 7 days. After compression and staining with Coomassie brilliant blue, a large, dark zone around the point of inoculation is indicative of twitching motility. Assays of wild-type strain VCS1703A (WT), the fimN2 mutant JIR3896, the fimO2 mutant JIR3886, the fimP2 mutant JIR3890, and the pilE2 mutant JIR3911 are shown. The profile obtained from the second mutant of each gene was identical to that of each mutant represented here.