FIG. 4.

Sequence alignment of FimA and pilin-like proteins and genetic organization of the pilCVWX-fimU-pilE gene cluster. (A) N-terminal sequence alignment. The arrow indicates the prepilin processing site of FimA and the predicted processing sites of the other proteins. Identical residues are shaded in black, while residues with strong and weak similarity are shaded in gray. The atypical threonine residue at +5 in PilX is boxed. (B) Genetic organization of the pilC to pilE cluster. The direction of transcription, predicted molecular sizes of the corresponding proteins, and clusters of orthologous groups (COG) as determined by an NCBI (http://www.ncbi.nlm.nih.gov) conserved domain search are shown. The genes are shown to scale.