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Two-dimensional gel electrophoresis of extracellular extracts of wild type (WT) and mutants. Culture supernatants were concentrated by TCA precipitation and subjected to two-dimensional gel electrophoresis. Sections of representative two-dimensional gels of the wild-type strain VCS1703A and the fimP2 mutant JIR3890 are shown. Proteins with reduced intensity in the mutant are circled and numbered from P1 to P6, as indicated.
