FIG. 7.

Complex formation between full-length RimM and S19. (A) Size exclusion chromatography, performed using a Superdex 75 10/30 column. The mixture of full-length RimM and S19 in the molar ratio of 1:2 is shown by the black line. Each chromatography step with full-length RimM (20 nmol; red) and S19 (40 nmol; cyan) was performed under the same conditions. Fraction numbers are indicated in the panel with the corresponding colors. (B) SDS-polyacrylamide gel electrophoresis of fractions collected from size exclusion chromatography in panel A. Fraction numbers are indicated at the top: fractions 3 (red number), 4 (cyan), and 1 to 6 (black) correspond to full-length RimM, S19, and their mixture, respectively. Molecular weight standards are displayed as “M.”