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DNA of sorghum BAC clone 52A4 was digested with restriction enzymes DraI (lane 1), SalI (lane 2), Sau3AI (lane 3), AccI (lane 4), and HindIII (lane 5). The same blot was probed with genomic DNA from wheat, maize, and rice, respectively. A 745 Sau3AI fragment (arrow) hybridized to the genomic DNA from all the three species. This fragment was subcloned as plasmid pSau3A9.
