TABLE 1.
Identified extracellular proteins of T. fusca grown on cellobiose
| Spot no. | Protein name (designation) | Accession no. | Theoretical Mr/pI | Exp Mr/pI | Random probabilitya | No. of peptides identified/% coverage | Locusb |
|---|---|---|---|---|---|---|---|
| 1 | β-1,4-Exocellulase (Cel48A) | YP_290015 | 104/4.2 | 110/3.3 | 5.03−12 | 24/13 | Tfu_1959 |
| 3 | 110/3.4 | 4.97−8 | 7/8.0 | ||||
| 4 | 90/3.3 | 1.04−10 | 18/12 | ||||
| 6 | 80/3.4 | 3.22−8 | 7/6.5 | ||||
| 21 | 27/4.2 | 1.33−9 | 2/2.7 | ||||
| 3 | β-1,4-Endocellulase (Cel9B) | YP_289685 | 101/4.0 | 110/3.4 | 3.27−13 | 8/9.8 | Tfu_1627 |
| 4 | 90/3.3 | 1.85−9 | 2/1.7 | ||||
| 5 | 82/3.4 | 7.23−11 | 4/6.6 | ||||
| 10 | 45/3.4 | 1.45−11 | 6/7.4 | ||||
| 2 | β-1,4-Endo/exocellulase (Cel9A) | YP_290232 | 90/4.4 | 110/3.7 | 4.8−12 | 11/16 | Tfu_2176 |
| 18 | 34/4.2 | 7.16−7 | 2/2.3 | ||||
| 8 | β-1,4-Exocellulase (Cel6B) | YP_288681 | 60/4.1 | 65/3.4 | 1.1−16 | 30/25 | Tfu_0620 |
| 11 | 44/4.1 | 1.02−10 | 24/30 | ||||
| 12 | β-1,4-Endoxylanase (Xyl10A) | YP_290979 | 49/4.9 | 45/4.7 | 2.74−14 | 23/51 | Tfu_2923 |
| 15 | 42/4.1 | 1.16−11 | 3/7.7 | ||||
| 14 | β-1,4-Endoglucanase (Cel5A) | YP_288962 | 50/5.2 | 45/4.6 | 4.06−6 | 4/8.4 | Tfu_0901 |
| 15 | Putative cellulose-binding protein (E8) | YP_289723 | 47/4.4 | 42/4.1 | 4.98−10 | 11/19 | Tfu_1665 |
| 13 | β-1,4-Endoglucanase (Cel6A) | YP_289135 | 46/5.9 | 45/5.3 | 1.67−12 | 9/20 | Tfu_1074 |
| 16 | 40/5.3 | 1.67−9 | 1/2.7 | ||||
| 19 | Putative secreted proteolytic enzyme containing NLPC_P60 domain (E9) | YP_289090 | 36/4.5 | 35/4.2 | 2.35−8 | 2/7.0 | Tfu_1029 |
| 24 | Putative secreted cellulose-binding protein (E7) | YP_289329 | 25/7.5 | 20/5.9 | 7.94−10 | 13/24 | Tfu_1268 |
| 25 | 20/6.1 | 1.00−30 | 13/65 | ||||
| 26 | 17/5.8 | 7.90−12 | 2/12 | ||||
| 27 | 14/3.8 | 1.91−5 | 1/3.6 | ||||
| 20 | Putative secreted protein containing lytic transglycosylase and goose egg white lysozyme domain (E10) | YP_288519 | 23/4.2 | 26/3.3 | 6.72−12 | 2/11 | Tfu_0458 |
| 7c | β-1,3-Glucanase (Lam81A) | YP_290186 | 82/5.0 | 70/4.4 | 1.0−30 | 52/33 | Tfu_2130 |
| 9c | Predicted secreted aminopeptidase (E11) | YP_290459 | 49/4.5 | 57/4.0 | 1.46−9 | 28/27 | Tfu_2403 |
| 17c | Putative secreted proteolytic enzyme containing NLPC_P60 domain (E12) | YP_290318 | 42/4.9 | 40/4.7 | 6.31−11 | 8/22 | Tfu_2262 |
| 22c | Putative secreted xylanase or chitin deacetylase (E13) | YP_290845 | 27/6.7 | 27/5.8 | 4.25−6 | 4/9.8 | Tfu_2789 |
| 23c | 27/6.1 | 1.13−6 | 6/18 |
a
The probability, assigned by BioWorks 3.2 software, of finding the identified peptides randomly.
b
The loci listed are ORF numbers in the T. fusca YX genome sequence NC_007333, from NCBI (http://www.ncbi.nlm.nih.gov/Genomes/).
c
Protein spots with comparable intensities on both cellobiose- and glucose-derived 2DE gels.