TABLE 3.
Fold changes of T. fusca gene expression in BMCC and cellobiose compared to expression in glucose
| Locusa | Gene product (protein designation) | Transcript ratiob in:
|
|
|---|---|---|---|
| Cellobiose | BMCC | ||
| Tfu_1627 | β-1,4-Endoglucanase (Cel9B) | 9.4-15 | 18-25 |
| Tfu_1074 | β-1,4-Endoglucanase (Cel6A) | 4.1-8.3 | 5.0-7.4 |
| Tfu_0620 | Nonreducing end-directed β-1,4-exocellulase (Cel6B) | 12-28 | 28-40 |
| Tfu_2176 | β-1,4-Endoglucanase (Cel9A) | 3.2-5.4 | 4.3-6.2 |
| Tfu_0901 | Endo-1,4-β-glucanase (Cel5A) | 1.3-2.7 | 2.1-3.7 |
| Tfu_1959 | Reducing end-directed β-1,4-exocellulase (Cel48A) | 2.5-4.9 | 2.1-4.3 |
| Tfu_1268 | Predicted cellulose-binding protein (E7) | 3.9-11 | 4.8-6.6 |
| Tfu_1665 | Predicted cellulose-binding protein (E8) | 6.3-15 | 8.8-13 |
| Tfu_0934 | Predicted ABC-type sugar transport system, periplasmic component for cellobiose/cellotriose | 24-40 | 15-22 |
| Tfu_0937 | β-Glucosidase (BglC) | 7.1-16 | 5.1-8.7 |
| Tfu_0938 | CelR | 3-13 | 1.3-1.9 |
| Tfu_2923 | β-1,4-Endoxylanase (Xyl10A) | 4.7-5.6 | 4.3-6.0 |
| Tfu_2788 | Putative β-1,4-endoxylanase | 0.64-1.4 | 0.25-0.37 |
| Tfu_1213 | β-1,4-Xylosidase (TFX11A/Xyl11A) | 0.35-0.58 | 0.27-0.38 |
| Tfu_2791 | β-1,4-Endoxylanase (Xyl10B) | 0.29-1.5 | 0.74-1.0 |
| Tfu_1612 | Putative secreted xyloglucanase | 0.69-1.5 | 0.82-1.5 |
| Tfu_2990 | Putative secreted xylanase | 0.82-1.7 | 0.43-0.65 |
| Tfu_0900 | Putative secreted β-mannanase | 0.75-1.6 | 1.1-1.5 |
a
The loci listed are ORF numbers in the T. fusca YX draft assembly genome sequence NC_007333, from NCBI (http://www.ncbi.nlm.nih.gov/genomes/).
b
Analysis of the microarray data with 95% confidence intervals using a Markov chain Monte Carlo (MCMC) method described in Materials and Methods showed that the expected transcript level changes in BMCC and cellobiose were between upper and lower bounds.