TABLE 2.
Primers used for cloning and RT-PCR
| Primer | Sequence (5′-3′) |
|---|---|
| LuxSSpeR | AAAATCATTCCCCAAACCAATGATCAGGG |
| LuxSEcoF | CCGGAATTCAGAGCAAGCGTTCGCTAAAA |
| TopoXbaF | GCTCTAGACTGAGATCATCCGCAACCAT |
| TopoBlgR | GAAGATCTTTGGCATGTCCATGTGATCT |
| FBglIIpKan+ | GAAGATCTTCCCGGGCGAACCATTTGAGGTGA |
| PFA3 | GCTCTAGACCCGGGTATAAGCCCATTTTCATGC |
| FLS2Bam | CGGGATCCGAGGCGGTTGCTTTTGTAGA |
| LuxS3AB | CGACATAACTGGCATGGTTGGCGAACGCTTGCTCTAAA |
| RLS2Xho2 | TTTATCTTCTCGAGCGCCCATGAATGTCTGAAGT |
| RdxA1F | TAAACGAGCGCCATTCTTG |
| RdxA1LR | GATTAAAAGCGCTTCAGCGTGAGGCGGTTGCTTTTGTAGA |
| RdxA2LF | CTTCAGACATTCATGGGCGATTCAACCACAGCATGCAAA |
| RdxA2R | CGATCAAGCATGCGATTTTA |
| FliaAF | TGCTTCGTTTCAACCATGAG |
| FliaAK | AAGCAACAACACCACCATCACAGGAAACAGCTATGACA |
| FliaBK | CTGGCCGTCGTTTTACTCGCGCATTTCTCAAATC |
| FliaBR | ACTCACACGATTAGGGCGTTT |
| HP0244AF | CGATGAGATTTATGCGTCCA |
| HP0244AK | CCTTATTTGAAGCGTTCCCACAGGAAACAGCTATGAC |
| HP0244BK | ACTGGCCGTCGTTTTACGAGAGCGAACAGGGTCAA |
| HP0244BR | GCTAACCCTAGGCCGTTACC |
| FlgMAF | GTTGTGGTTGCTCATGTTCG |
| FlgMAK | AATGCCGTTTCTTCTCTTGCAGGCAACAGCTATGA |
| FlgMBK | ACTGGCCGTCGTTTTACTCTCACAAAATGGCAAAGGA |
| FlgMBR | TCCTAACTTCATGATTGA |
| FlhAIntF | CCTAGTGCGGTGAGCGATATTATC |
| FlhAIntR | GCTCACGCTAAATTCCCCAA |
| UreAIntF | AAAGACTGCGGCTGAATTG |
| UreAIntR | CCATCACATCATCCGGTTT |
| FlhABgl2 | GAAGATCTACCTCACAAGAACCCGTATCGT |
| FlhAXho2 | ATTCCGCTCGAGCAATGGAAACTCTCAGCCACTTC |
| M13Nhe1 | CTAGCTAGCGTAAAACGACGGCCAGT |
| M13Acs1 | TTGGCGCGCCCAGGAAACAGTTATGAC |
| 1320f | CCATGAAGTCGGAATCGCTAG |
| 1431r | ACTCCCATGGTGTGACGG |