FIG. 2.
Autoradiography of lysates of l-[35S]cysteine-labeled E. coli BL21 ihfA ihfB cells harboring the pET27b+ plasmid with Gifsy-1 xis cloned in frame to the Ptac promoter. The lanes of the 10 to 20% gradient tricine SDS-PAGE gel contain lysates of cells at an optical density at 600 nm of 0.6 (lanes 1), incubated in the absence of IPTG (A) or with 1 mM IPTG (B) for 15 min (lanes 2), incubated for 15 min after addition of rifampin (lanes 3), after incubation was continued for 1 h (lanes 4), and after incubation was continued overnight (lanes 5).