FIG. 4.

ChIP analysis of AtoC binding to atoDAEB promoter fragments in vivo. The binding of AtoC to plasmid-borne atoDAEB promoter fragments that either carry (atoD1) or lack (atoD2) the predicted binding site of AtoC (filled oval) in the presence (+) and absence (−) of acetoacetate (inducer) was determined. The atoSC⁺ strain BW25113 and its ΔatoSC isogenic counterpart BW28878, carrying either the plasmid pMLB-atoD1 or pMLB-atoD2 (Table 1), were used in this experiment. Immunoprecipitations were performed in the absence (−) or presence of anti-AtoC (α-AtoC/Az) antibodies, and they were followed by PCR amplification of the immunoprecipitated DNA fragments by using one atoDAEB-specific (U2patoD [Table 1]) and one plasmid-specific (universal M13 forward) primer. Chromatin input controls are indicated.