FIG. 3.

Silver-stained SDS-PAGE gels of 10-μl samples of HAP pools. Panels A and B correspond to HAP pools from independent experiments (whose results are shown in Fig. 2A and Fig. S2B in the supplemental material, respectively). The amount of SsbA and SsbB in reference lanes was 200 ng. For silver staining, gels were fixed in 40% ethanol-0.05% formaldehyde solution and incubated in 0.02% Na₂S₂O₃, followed by incubation in 0.1% AgNO₃ and visualization in 3% Na₂CO₃-0.02% Na₂S₂O₃-0.05% formaldehyde solution, as described previously (12). M, MM standards.