FIG. 1.

SDS-PAGE (10%) and Western blotting of C. trachomatis MoPn EB and COMC. (A) Coomassie blue R stain of 10% SDS-PAGE of C. trachomatis MoPn EB. The EB were incubated at room temperature or were boiled in the presence or absence of 25 mM DTT as indicated at the top of the lanes. (B and C) Western blot of same samples probed with mAb MoPn-13-2 (B) or with mAb MoPn-18b (C). (D) The COMC was extracted from EB with 2% Sarkosyl, and the insoluble pellet was resuspended in PBS buffer and incubated at room temperature or boiled in the presence (25 mM) or absence of DTT, as indicated. (E) Western blot of the same boiled, or nonboiled, COMC preparations in the presence of 25 mM DTT probed with mAb MoPn-13-2 to the MOMP, mAb MoPn-32 to LPS, and mouse polyclonal antibodies to the 60- and 12-kDa CRPs as indicated. MW standards (MW STDs) (in thousands) were loaded onto the first lane of each gel as shown.