FIG. 2.

SDS-PAGE (5%) and Western blotting of cross-linked C. trachomatis MoPn COMC. The COMC was cross-linked using BS³, or not cross-linked, and mixed with 2× sample buffer containing 50 mM DTT. Half of the preparation was boiled, and the other half was incubated at room temperature. MW standards (MW STDs) (in thousands) are shown in the first lanes of all the gels as marked. (A) Silver stain gel. The following antibodies were used: mAb MoPn-13-2 (B), mAb MoPn-18b (C), polyclonal serum to the 60-kDa CRP (D), and polyclonal serum to the 12-kDa CRP (E). Dot blots of the cross-linked and non-cross-linked proteins, probed with their respective antibodies, are shown on the top of each gel.