SDS-PAGE (10%) and Western blotting of purified C. trachomatis MoPn MOMP. (A) Coomassie blue G staining. (B and C) Western blot probed with mAb MoPn-13-2 (B) and mAb MoPn-18b (C). The samples were incubated at room temperature or boiled in the presence (25 mM) or absence of DTT as indicated on the top of each lane. Tc, compact trimer; Tr, relaxed trimer; M, monomer; Mc, compact monomer; MW STDs, MW standards (in thousands).