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Western blot of an 8% SDS-PAGE gel of the C. trachomatis MoPn MOMP cross-linked with DSP and probed with mAb MoPn-13-2. The first lane shows MW standards (MW STDs) (in thousands). The MOMP was cross-linked at different molar ratios with DSP (as labeled on the top of the lanes) or not-cross-linked and boiled or not boiled in 2× buffer without DTT, as indicated for each lane. T, trimer; Tc, compact trimer; D, dimer; M, monomer; Mc, compact monomer.
