FIG. 9.

Immunoblot of the MOMP incubated at various temperatures. The MOMP was incubated at different temperatures for 1 h, except for 10 min at 100°C, and was separated (1 μg/lane) on a 10% SDS-PAGE gel before it was probed with mAb MoPn-13-2 (A, C, and E) and mAb MoPn-18b (B, D, and F). The first lanes show MW standards (in thousands). (A and B) Samples incubated without SDS or DTT. (C and D) Samples incubated with 2% SDS without DTT. (E and F) Samples incubated with 2% SDS and 25 mM DTT.