TABLE 3.

Distribution of genes related to replication, sugar metabolism, and bacteriocin production on the repA-type megaplasmids in 33 strains of L. salivarius^a

repA-type megaplasmid	Strain	Size of megaplasmid (kb)	LSL_1739 (repA)	LSL_1740 (repE)	LSL_1741 (parA)	Sorbitol utilization		Rhamnose utilization		Ribose utilization			Bacteriocin production
						Phenotype	LSL_1894	Phenotype	LSL_1752 (rhaB)	Phenotype	LSL_1888 (mipB)	LSL_1946 (tktA)	Phenotype	LSL_1916 + LSL_1917 (Abp118α + Abp118β)
pMP20555	DSM20555	380b	+	+	+	F	+	F	+	NF	+	+	NBA	N
pMP14476	LMG14476	290	+	+	+	F	+	F	+	NF	+	+	NBA	+
pMP14477	LMG14477	270	+	+	+	F	+	F	+	NF	+	+	NBA	+
pMP20554	DSM20554	260b	+	+	+	F	+	NF	N	NF	+	+	NBA	N
pMP118	UCC118	242b	+	+	+	F	+	F	+	F	+	+	BA	+
pMP43310	AH43310	240	+	+	+	F	+	F	+	F	+	+	BA	+
pMP43324	AH43324	240	+	+	+	F	+	F	+	F	+	+	BA	+
pMP20492	DSM20492	240b	+	+	+	F	+	WF	+	NF	+	+	NBA	N
pMP44481	CCUG44481	240	+	+	+	F	+	NF	N	NF	+	N	NBA	+
pMP1047	JCM1047	240	+	+	+	F	+	NF	N	NF	+	N	NBA	+
pMP47171	CCUG47171	240	+	+	+	F	+	F	+	NF	+	+	NBA	+
pMP1046	JCM1046	230	+	+	+	F	+	F	+	NF	+	+	NBA	+
pMP1045	JCM1045	220	+	+	+	F	+	F	+	NF	+	+	NBA	+
pMP47825	CCUG47825	220	+	+	+	F	+	F	+	WF	+	+	NBA	+
pMP45735	CCUG45735	220	+	+	+	F	+	F	+	WF	+	+	NBA	+
pMP27530B	CCUG27530B	220	+	+	+	F	+	NF	+	WF	+	+	NBA	+
pMP47826	CCUG47826	220	+	+	+	F	+	F	+	NF	+	+	NBA	+
pMP43299	CCUG43299	218	+	+	+	F	+	NF	N	WF	+	+	NBA	N
pMP38008	CCUG38008	215	+	+	+	F	+	NF	N	WF	+	N	NBA	+
pMP4231	AH4231	210b	+	+	+	F	+	NF	+	NF	+	+	BA	+
pMP4331	AH4331	210	+	+	+	F	+	NF	+	NF	+	+	BA	+
pMP14315	01M14315	200	+	+	+	F	+	NF	N	NF	+	+	NBA	N
pMP119	UCC119	195b	+	+	+	NF	N	F	+	NF	N	N	BA	+
pMP43348	AH43348	195	+	+	+	F	+	NF	+	WF	+	+	ND	+
pMP8818	NCIMB8818	195	+	+	+	F	+	WF	+	NF	+	+	NBA	+
pMP1040	JCM1040	195	+	+	+	NF	N	NF	N	NF	+	+	NBA	N
pMP21	L21	190	+	+	+	F	+	F	+	NF	+	+	NBA	+
pMP8816	NCIMB8816	180b	+	+	+	F	+	NF	N	NF	+	N	NBA	N
pMP1042	JCM1042	180	+	+	+	F	+	NF	N	NF	+	N	NBA	N
pMP1044	JCM1044	180	+	+	+	F	+	NF	N	NF	+	N	NBA	N
pMP702343	NCIMB702343	160b	+	+	+	NF	N	WF	+	NF	N	N	NBA	+
pMP8817	NCIMB8817	145b	+	+	+	NF	N	NF	+	NF	N	+	NBA	N
pMP1230	JCM1230	100b	+	+	+	F	+	NF	N	NF	+	+	NBA	+

^a+, gene detected by hybridization; N, no hybridization signal detected; F, carbon source fermented; WF, carbon source weakly fermented; NF, carbon source not fermented; BA, bacteriocin activity detected; NBA, no bacteriocin activity detected; ND, not determined. Boldface type indicates that the gene is present but has a conflicting phenotype; italic type indicates that the gene is absent and has a corresponding phenotype. Data for carbohydrate-fermenting tests were obtained from our previous study (38). Indicator strains used in this study for determining the bacteriocin activity were L. monocytogenes EGDe and L. sake LMG2313.

^bThe size of these repA-type megaplasmids were estimated in a previous study (13).