Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Heme-dependent expression of heme protein polypeptides. B. japonicum cytochrome c550, E. coli cytochrome b562, and Vhb were expressed from a plasmid as His-tagged proteins in E. coli hemA strain S905 grown in medium containing either 60 μM (L) or 600 μM (H) ALA. Cells that expressed B. japonicum cytochrome c550 also harbored the ccm genes on pEC86. Cell extracts were analyzed by immunoblotting using antibodies against the His tag. Thirty micrograms of protein was loaded per lane.
