FIG. 2.

The capacity of a ΔtcpH ΔyaeL double mutant of V. cholerae to degrade TcpP is restored by introduction of hurP. V. cholerae O395, O395ΔtcpH, and O395ΔtcpHΔyaeL, each transformed with pBAD18-Kan, pBAD18-Kan-yaeL (yaeL), or pNATX12.1 (hurP), were cultured in LB broth (pH 6.5) at 30°C in the presence of 0.1% arabinose. Mid-logarithmic-phase cells were solubilized, the proteins were resolved by SDS-PAGE, and the resolved proteins were analyzed by immunoblotting with anti-TcpP antibodies. Protein standard molecular sizes are in kilodaltons. (Strains of V. cholerae, anti-TcpP antibodies, pBAD18-Kan, and pBAD18-Kan-yaeL were obtained from J. S. Matson and V. J. DiRita.)