FIG. 2.

Impaired neuronal architecture in FcγRIIB-deficient and CD3ɛ-deficient mice during cerebellar development. (A) RT-PCR analysis of each CD3 subunit in the EL4 T-cell line and the cerebellum from C57BL/6 mice at P1, P21, and the adult stage. β-Actin was used as the internal control. (B) Triple immunofluorescence for GLAST (red), calbindin (green), and NeuN (blue) at P7. Arrows and arrowheads indicate migrating GCs with an ellipsoidal shape and those with a round shape, respectively. (C) Immunofluorescence for NeuN at P3 and P7. The relative area occupied by granule cells was quantitatively compared. (D) Immunofluorescence for calbindin at P7. (E) The pixel intensity of VGLUT1 immunofluorescence was quantitatively compared. The left panel shows representative examples of immunofluorescence staining with anti-vGluT1 antibody at P7. The images were taken at the same exposure. Asterisks, Purkinje cell somata. Bars, 10 μm.