FIG. 2.

FISH analyses of nuclear localization of the TNF-α locus. The THP-1 and K562 cell lines are triploid for chromosome 6. The confocal panels show representative cells. Centromere probes are shown in green (FITC), and the TNF-α locus is shown in red (Spectrum orange). The graph shows the cumulative findings from 10 to 20 cells. (A) K562 cells. One locus is in heterochromatin in the upper right corner. (B) Jurkat cells. (C) THP-1 cells. All three loci are in euchromatin. (D) Graphical representation of the proximity of the TNF-α loci to the closest centromeric heterochromatin. “THP-1-LPS” refers to THP-1 cells treated with 1 μg/ml of LPS. A distance of ≤0.3 μm is considered localization to heterochromatin.