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DNA methylation status of the TNF-α locus in primary cells. The legend is as for Fig. 1. Cell sources were undifferentiated hESCs, hEBs harvested at various times in culture (+, growth in cytokine-supplemented media), hematopoietic stem cells (HSC) purified from a peripheral blood harvest, human liver, peripheral blood monocytes purified by elutriation, and peripheral blood monocytes differentiated by adherence for 7 days into macrophages.
